Standard Operating Procedure

DATE: May 2007

TYPE OF RESEARCH: Microfluidics
HAZARDS: Laser radiation (Class IIIb), Ultra Violet Light

PROTECTIVE CLOTHING THAT MUST BE USED FOR ALL WORK:

• Safety Goggles - Must be worn when working with any chemicals that could cause eye damage. Check the MSDS section on protective clothing for each chemical in use. The MSDS are on the web at (http://www.uos.harvard.edu/cgi-bin/msds/msds_bl.pl) and some are also placed in the cabinet in ESL 215.
• Aprons - Should be worn when working with corrosives such as strong acids or bases. Check the MSDS section on protective clothing for each chemical in use.
• Gloves - Must be worn when working with any chemicals or cryogens. Proper glove selection is critical. Check the MSDS for all chemicals in use. At the Safety Committee web site (http://www-safety.deas.harvard.edu) under "Safety Committee Services" can be found a listing of glove type vs. chemical. If you have any questions, contact your local safety officer.
• Laser Safety goggles - It is generally safe, as it is difficult to get your eye in the path of the laser beam, but it is recommended that you use safety goggles when any alignment procedure is being done.

1. Be respectful of other people when using shared facilities and clean up after yourself.
2. Return borrowed tools ASAP to the tool chest (located in Room 214) from which they came.
3. Any glassware should be cleaned and hung to dry and/or put away in the proper place before leaving the room.
4. Clutter should be avoided.

1. All glass should be disposed in an appropriate Broken Glass Waste Box.
2. ALL sharps (including razor blades and syringes (with or without needles) are considered a biohazard and must be disposed of in an appropriate Sharps Collection container.
3. Everyone must have current Hazardous Waste Disposal training which includes the initial "live" training and annual "on line" refresher training. 
4. All chemical waste must have a university provided Hazardous Waste Tag on it. The tag should be properly filled out with all of the information except the date when the container first has waste put into it. The date is filled in when the container becomes FULL. Writing on the container itself is not acceptable.
5. Satellite Accumulation Areas are located in the fume hood in ESL 215. All Hazardous Waste requires secondary containment. Do not store incompatible chemicals (i.e. acids and bases) in the same secondary containment tray and should be stored in the SAA. Do not store incompatible chemicals (i.e. acids and bases) in the same secondary containment tray.
6. When a waste bottle is filled, the Hazardous Waste Tag must be DATED and EH&S must be called at once for waste pick-up. The number to call is on the green SAA sign posted in the area; alternatively, a pickup request can be made at https://www.uos.harvard.edu/cgi-bin/ehs/ehs-bin/hw_services.pl.

• Chemicals should be stored in the appropriate safety cabinet (flammables, acids, bases) in ESL 215. No chemical should be left in a fume hood unless actively in use. The exception is Hazardous Waste, which may be stored in a fume hood designated for this purpose (with a green SAA sign).
• All vials must be labeled and kept in the personal storage cabinets. The optical table is NOT meant for long-term storage of samples.

1. All lab personnel who work with chemicals should have Chemical Safety training.
2. All lab personnel who generate Hazardous Waste must have current Hazardous Waste Disposal training which includes the initial "live" training and annual "online" refresher training which is located through http://www.uos.harvard.edu.
3. All persons working with lasers should have Laser Safety training.
4. All lab personnel who work with compressed gases should take the Compressed Gas Safety training.
5. All personnel who would like to use the Physics Machine Shop must take the Machine Shop course with Stan Coutreau, which includes machine shop safety training.
6. All of the above mentioned training is offered annually through the SEAS Safety Committee.

CHEMICAL SPILL CLEAN-UP PROCEDURES:

1. Small spills should be cleaned up by the lab members. There are Spill Kits located in opposite Room 218 (Wall Closet).
2. For large spills, clear the area of personnel and call the EH&S control center at 5-2852.

1. Do not attempt to use ANY equipment that you are not trained to use.
2. Any room with HF in it must have Calcium Gluconate easily accessible in the same room. Be sure to check the expiration date and replace as necessary. Calcium Gluconate is available in the Chemistry or Biology stockrooms.
3. Basic medical kit is near the yellow cabinet in the 2nd floor (hallway in front of the elevator).
4. The Eye Wash is located by the sink in ESL 214.
5. The Safety Shower is located in front of ESL 216A.
6. If you have any questions ask your local safety officer.